Qualitative Determination of Proanthocyanidin Cleavage Products After Acid-Catalyzed Degradation in the Presence of Excess Phloroglucinol by Cyclodextrin-Modified Micellar Electrokinetic Chromatography

Abstract

A micellar electrokinetic chromatographic (MEKC) method has been established for the identification and determination of acid-catalyzed proanthocyanidin cleavage products in the presence of phloroglucinol. Using fused-silica capillaries (50/57 cm, 22 kV) with phosphate buffer containing sodium dodecyl sulfate as the surfactant and β-cyclodextrin as modifier, structurally similar phloroglucinol adducts and flavan-3-ols were fully separated.

Keywords:

• Capillary electrophoresis
• Cistus albidus
• cyclodextrin-modified micellar electrokinetic chromatography (MEKC)
• phloroglucinol
• proanthocyanidin