Prevention of seminal vesicle damage by Mucuna pruriens var. pruriens seed extract in chronic unpredictable mild stress mice

Abstract

Context

Thai Mucuna pruriens (L.) DC. var. pruriens (Fabaceae) or T-MP seed extract has been shown to improve sexual performance and sperm quality.

Objective

This study investigates the preventive effects of T-MP against seminal vesicle damage, apoptotic and Nrf2 protein expression in mice under chronic unpredictable mild stress (CUMS).

Materials and methods

Forty-eight male ICR mice were divided into four groups: control, CUMS, T-MP300 + CUMS and T-MP600 + CUMS. Mice in control and CUMS groups received distilled water, while those in treated groups were pretreated with T-MP extract (300 or 600 mg/kg BW) for 14 consecutive days. The CMUS and co-treated groups were exposed to one random stressor (of 12 total) each day for 43 days. Components and histopathology of the seminal vesicle were examined, along with localization of androgen receptor (AR) and caspase 3. Expression of seminal AR, tyrosine phosphorylated (TyrPho), heat shock protein 70 (Hsp70), caspases (3 and 9) and nuclear factor erythroid 2-related factor 2 (Nrf2) proteins was investigated.

Results

T-MP extract at a dose of 600 mg/kg BW improved seminal epithelial damage and secretion of fluid containing essential substances and proteins in CUMS mice. It also increased the expression of AR and TyrPho proteins. Additionally, T-MP increased expression of Nrf2 and inhibited seminal vesicular apoptosis through the suppression of Hsp70 and caspase expression.

Conclusion

T-MP seeds have an antiapoptotic property in chronic stress seminal vesicle. It is possible to apply this extract for the enhancement of seminal plasma quality.

Keywords:

• Seminal plasma
• CUMS
• apoptosis
• Nrf2
• TyrPho

Acknowledgments

We would like to thank associate professor Dr. Sitthichai Iamsaard, Department of Anatomy, Faculty of Medicine, Khon Kaen University for providing histological and biochemical apparatuses. In addition, we also acknowledge Dr. Dylan Southard for editing this MS via the KKU Publication Clinic (Thailand).

Disclosure Statement

The authors have no conflict of interest to declare.

Additional information

Funding

This work was financially supported by the Walailak University Research Institute for Health Science (Thailand; Grant No. WU-IRG-65-012) to Dr. Pannawat Choowong-In.