Da-Yuan-Yin decoction polyphenol fraction attenuates acute lung injury induced by lipopolysaccharide

Abstract

Context

Da-Yuan-Yin is a Chinese traditional prescription.

Objective

This study explores the therapeutic effects of the Da-Yuan-Yin decoction polyphenol fraction (DYY-4) on acute lung injury (ALI) in mice induced by lipopolysaccharide (LPS).

Materials and methods

The mice (n = 10) were orally administrated with DYY-4 (15, 30, and 60 mg/kg) or DXM (5 mg/kg), half an hour after LPS (2 mg/kg) instilled intratracheally. The protein content and the levels of inflammatory factors, the levels of complements, the mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the level of myeloperoxidase (MPO) and superoxide dismutase (SOD), the expression of the IkB kinase (IKK) and nuclear factor-kappa B (NF-κB), the lung wet-to-dry weight (W/D) ratio and lung tissue were evaluated, 24 h after LPS challenge. Network pharmacology predicted potential targets.

Results

DYY-4 (30, 60 mg/kg, p < 0.01, p < 0.01) decreased the lung W/D ratio, total protein concentration, the levels of C3, C3c and C5a, the levels of TNF-α, IL-6, and IL-1β, while increased the levels of IL-4 and IL-10. DYY-4 (60 mg/kg) decreased the levels of C5aR1, C5b-9 and COX-2 mRNA (p < 0.05), the levels of MPO and iNOS mRNA, the activation of the IKK/NF-κB pathway (p < 0.01), and increased the levels of IL-13 and SOD (p < 0.01). DYY-4 (60 mg/kg) relieved the lung tissue pathological changes and reduced the C3c deposition.

Discussion and conclusions

Network pharmacology combined with animal experiments revealed the targets of DYY-4 alleviating ALI.

Keywords:

• Complement
• oxidase
• inflammatory factors
• NF-κB
• IKK
• network pharmacology

Acknowledgements

The authors acknowledge Faiz Ur Rehman from Soochow University and Dun-fang Wang from the Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences for their help to improve the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by Science and Technology Department of Suzhou, China under Grant [No. SYS2020079], Jiangsu Health Commission Medical Research Projects, China under Grant [No. Z2020063] and 2018 Qinglan Project of the Yong and Middle-aged Academic Leader of Jiangsu College.