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Summary
The feasibility and anatomical development of an in vitro olive cleft-graft method were studied. Grafting survival after 60.d in vitro was 85% which then dropped slightly to 67% after hardening. Three days after grafting, callus formation was observed along the cut surfaces of the rootstock and scion, after 6.d the first healing cellular unions were observed, and by 12.d after grafting a strong union developed. The first cellular differentiation to form vascular tissues was observed 12.d after grafting and continued rapidly until a total connection was reached 10.d later. Thus a complete graft union of the in vitro olive cleft-grafts was achieved in three weeks, and vigorous plants were established after 60.d of in vitro culture followed by 10.d of hardening. The in vitro olive cleft graft is suggested as an effective and useful method for germplasm multiplication.
