Induced androgenesis as a biotechnology method for obtaining DH plants in Daucus carota L.

ABSTRACT

The influence of various factors on the efficiency of obtaining double haploid lines in anther cultures was examined in Daucus carota L. The impact of the genotype, the formation of donor plants and the growth conditions was investigated. The effects of various regeneration media were analysed. Acclimatisation of androgenic plants and their evaluation were also conducted. Androgenesis was induced on B₅ medium containing 2,4-D and NAA at 0.1 g·L⁻¹. Depending on the genotype, 1.2–305.3 embryos per 100 anthers were obtained. The highest number of embryos (5.5 per 100 anthers) was obtained from donor plants with the first-order shoots containing one umbel. Cultivation of donor plants under controlled conditions improved the number of embryos from 305 in the open field to 1764 in the growth chamber. Regeneration of plants from androgenetic embryos proceeded most effectively on B₅ medium without hormones and amino acids. Regenerated plants adapted in over 60%. Ploidy analysis showed the presence of 92% of plants with a doubled chromosome set and 8% with a tetraploid number of chromosomes. Plants with a doubled chromosome set were 73% homozygous for the glucose phosphate isomerase isoenzyme and 100% homozygous for the aspartate aminotransferase isoenzyme, which confirms their gametic origin.

KEYWORDS:

• androgenesis
• anther culture
• carrot
• homozygosity
• ploidy

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the European Union, Programme Innovative Economy under Grant: UDA-POIG.01.03.01-10-114/08-05. New technology for deriving starting materials for breeding F₁ hybrids of carrot; European Union Agency for Network and Information Security.