ABSTRACT
Molecular markers are helpful to efficiently protect the plant breeders’ rights. Fluorescent simple sequence repeats (SSR) based on DNA sequencing is a powerful and automated platform for the detection of genetic polymorphism. Here, we first reported 13 SSRs using a fluorescent SSR panel according to the reference genome information, and detected the genetic diversity and population structure of 136 pomegranate varieties. The average number of alleles per locus was 6.31, gene diversity ranged from 0.16 to 0.37 with an average of 0.28, and polymorphic information content (PIC) varied from 0.14 to 0.29 with a mean value of 0.22. The mean genetic distance (GD) between varieties was 0.32. Population structure analysis divided the varieties into three different subpopulations (Q1, Q2, and Q3). The cultivars in Q1 all came from China, and almost all cultivars came from foreign countries combined with Chinese were grouped into Q2. The only accession originated from Myanmar was grouped to Q3. The results indicated that 13 SSR markers were polymorphic. The varieties originating from the same geographical region were more closely related, and extensive gene flow had taken place between the varieties from different regions. The results may be useful for the efficient selection breeding and variety protection.
Highlights
A total of 13 SSRs were first reported by using a fluorescent SSR panel according to the reference genome information.
The genetic diversity and population structure of 136 pomegranate varieties were detected with the 13 SSRs.
Genetic diversity analysis indicated the 13 SSRs were polymorphic and reliable.
Population structure analysis revealed the genetic difference between subpopulations.
Acknowledgments
This work was supported by a grant from The Investigation and Collection of Indigenous Varieties of Deciduous Fruit Trees in Predominance Region (2012 FY110100), The Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2015-ZFRI) and Agglomeration Project from Zaozhuang City of China (2016No23).
Disclosure statement
No potential conflict of interest was reported by the authors.
Supplementary material
Supplemental data for this article can be accessed here.