Jasmonic acid-induced metabolic responses in bitter melon (Momordica charantia) seedlings under salt stress

ABSTRACT

In the present study, effects of foliar application of 150 µM Jasmonic acid (JA), an endogenous plant growth regulator, on alleviation oxidative stress resulted from without (0 mM), low (50 mM), moderate (150 mM), and high (300 mM) NaCl in bitter melon (Momordica charantia) seedlings was investigated. High salt stress significantly decreased growth components including root length, leaf morphological properties, leaf water content, plant biomass and levels of pigments, soluble sugars, starch and proteins, but accumulated higher amounts of proline, free amino acids and the activities of ascorbate peroxidase (APX), catalase (CAT), polyphenol oxidase (PPO) and pyrogallol peroxidase (PPX). Exogenous JA treatment alone or combined with salt stress improved growth components with hyperaccumulation of soluble sugars, proteins, amino acids, proline and PPO activity, but was associated with a decrease in the activity of H₂O₂-decomposing enzymes. These results suggest that exogenous JA treatment can effectively raise salt stress tolerance of bitter melon seedlings through modifying allocation of carbon and nitrogen metabolism to adjust levels of metabolites and directing them to the growth processes.

KEYWORDS:

• Bitter melon
• Jasmonic acid
• proline
• reducing sugar
• salt stress

Acknowledgments

We thank the USB Deputy of Research for financial supports in the form of grants to S. Alisofi’s MSc research project.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the University of Sistan and Baluchestan;