Monitoring hepatitis B by using point-of-care testing: biomarkers, current technologies, and perspectives

ABSTRACT

Introduction: Liver diseases caused by hepatitis B virus (HBV) are pandemic infectious diseases that seriously endanger human health, conventional diagnosis methods can not meet the requirements in resource-limited areas. The point of acre detection methods can easily resolve those problems. Herein, we review the most recent advances in POC-based hepatitis B detection methods and present some recommendations for future development. It aims to provide ideas for future research.

Areas covered: Epidemiological data on Hepatitis B, conventional diagnostic methods for hepatitis B detection, some latest point of care detection methods for hepatitis B detection and list out the recommendations for future development.

Expert opinion: This manuscript summarized traditional biomarkers of different hepatitis B stages and recent-developed POCT platforms (including microfluidic platforms and lateral-flow strips) and discuss the challenges associated with their use. Some emerging biomarkers that can be used in hepatitis B diagnosis are also listed. This manuscript has certain guiding significance to the development of hepatitis B detection.

KEYWORDS:

• Hepatitis B
• epidemiological
• biomarker
• point of care
• lateral flow
• microfluidics

Article Highlights

• The epidemiological data of hepatitis B were analyzed.

• Summarized the conventional and latest biomarkers for hepatitis B

• The demand of POC platforms was analyzed.

• Summarized the latest POC platforms for hepatitis B detection.

• The future development of POC platforms for hepatitis B detection is prospected.

Declaration of interest

The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

Reviewer Disclosures

Peer reviewers on this manuscript have no relevant financial or other relationships to disclose.

Acknowledgments

We also thank Miss Yexin Yang (School of Life Science and Technology, Xi’an Jiaotong University) for helping to proofread the manuscript for grammar.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China (No. 51801001), China Postdoctoral Science Foundation (No. 2016M601878), Provincial Key Research and Development Program of Shaanxi (No. 2019GY-197), Key Project of Baoji University of Arts and Sciences (No. ZK2018051), the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2015JM5215), Baoji University of Arts & Sciences postgraduate innovative research projects (YJSCX19ZD04, YJSCX18YB36). AL Feng received funding from The Thousand Talents Plan for Young Professionals of Shaanxi Province and High-level Leading Talents of Scientific and Technological Innovation of Baoji. The funding agencies had no role in the study design, the collection, analysis, or interpretation of data, the writing of the report, or the decision to submit the article for publication.