Inhibition of a thermophilic deoxyribonucleic acid polymerase by fullerene derivatives

Abstract

Enzyme inhibition by fullerene derivatives has attracted much attention. In this communication, effects of two water-solube fullerene derivatives, fullerol and trimalonic acid C₆₀ (TMA C₆₀) on polymerase chain reaction (PCR) were investigated by using PCR of β-actin cDNA derived from HeLa cells as an experimental model. Both fullerol and TMA C₆₀ were found to inhibit PCR in a dose-dependent manner. PCR was ultimately inhibited while the concentrations of each compound were not less than 0.01 mM. In contrast, mannitol exerted no effects on PCR while its concentration increased up to 2 mM. Compensation experiments with Thermus aquaticus (Taq) DNA polymerase revealed that both fullerol and TMA C₆₀ inhibited the enzymatic activity of Taq DNA polymerase, and the inhibitory potency of TMA C₆₀ was slightly greater than that of fullerol. Our data provides some novel aspects on the enzyme inhibiting activities of fullerene derivatives.

Keywords::

• Fullerol
• trimalonic acid C₆₀
• Taq DNA polymerase
• inhibition

Acknowledgements

This work was supported by Specialized Research Fund for the Doctoral Program of Higher Education (No. 20030007011) and Basic Research Foundation of Beijing Institute of Technology (No. 200306C02).