The leaf extract of Siberian Crabapple (Malus baccata (Linn.) Borkh) contains potential fatty acid synthase inhibitors

Abstract

The present work focused on the kinetics of the inhibitory effects of the leaf extract of Siberian Crabapple, named Shan jingzi in China, on chicken liver fatty acid synthase. The results showed that this extract had much stronger inhibitory ability on fatty acid synthase than that from green teas described in many previous reports. The inhibitory ability of this extract is closely related to the extracting solvent, and the time of extraction was also an important influencing factor. The inhibitory types of this extract on diffeerent substrates of chicken liver fatty acid synthase, acetyl-CoA, malonyl-CoA and NADPH, were found to be noncompetitive, uncompetitive and mixed, respectively. The studies here shed a new light on the exploration for inhibitors of fatty acid synthase.

Keywords::

• Siberian Crabapple
• leave extract
• fatty acid synthase (FAS)
• inhibitor

Acknowledgements

This work was supported by the National Natural Science Foundations of China (30670460, 30770475), the Science and Technology Planning Project of Zhejiang Province of China (2007C24021), the Science and Technology Planning projects of Jiaxing, Zhejiang, China (2007AZ1015, 2007A36) and the Qianjiang Program of Zhejiang Province, China (2006R10036).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.