Amniotic fluid CD11b levels in pregnancies complicated by preterm prelabor rupture of membranes

Abstract

Objective

CD11b is an integrin molecule located on the surface of leukocytes. CD11b is involved in the processes of cell adhesion and migration. Expression of CD11b increases during inflammation. Therefore, this study was aimed at the evaluation of concentrations of CD11b in the amniotic fluid from pregnancies complicated by preterm prelabor rupture of the membranes (PPROM), with respect to the presence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI (the presence of both MIAC and IAI).

Methods

Eighty women with singleton pregnancies complicated by PPROM were included. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid CD11b concentrations were determined by enzyme-linked immunosorbent assay. MIAC was determined by a non-cultivation approach. IAI was defined by a bedside amniotic fluid interleukin-6 concentration ≥745 pg/mL.

Result

Women with MIAC or microbial-associated IAI had higher CD11b concentrations in the amniotic fluid than women without these complications (with MIAC: median 0.31 ng/mL versus without MIAC: median 0.17 ng/mL, p = .001; with microbial associated-IAI: median 0.35 ng/mL versus without microbial-associated IAI: median 0.16 ng/mL; p =.02). The presence of IAI was not associated with elevated CD11b concentrations. A weak negative correlation was found between amniotic fluid CD11b concentrations and interleukin-6 concentrations (rho = 0.26; p = .02).

Conclusions

MIAC and microbial-associated IAI are characterized by higher amniotic fluid CD11b concentrations in pregnancies complicated by PPROM.

Keywords:

• Amniotic fluid
• intergrin
• inflammation
• preterm delivery

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was supported by Charles University in Prague, Faculty of Medicine in Hradec Kralove, Czech Republic, project “PROGRES P40/10,” Faculty Hospital in Hradec Kralove (long-term organization development plan), and the project PERSONMED – Center for the Development of Personalized Medicine in Age-Related Diseases, Reg. Nr.CZ.02.1.01/0.0/0.0/17_ 048/0007441.