Abstract
On two small islands of the Tuscan Archipelago, two new endemic species of the Saxifraga granulata group, namely S. caprariae and S. montis-christi, were recently described on a morphological basis. This led us to investigate these species using a molecular approach involving the analysis of ITS and trnL-F regions. Our aims were: to highlight their phylogenetic traits; to confirm or reject the hypothesis that they are more closely related to S. corsica than to S. granulata; and to verify their phylogenetic position in the context of Saxifraga L. ser. Saxifraga. We considered all the species belonging to the ser. Saxifraga and some other species of sect. Saxifraga – a total of 19 species. We generated 10 new DNA sequences, six for ITS and four for trnL-trnF. We produced separate Bayesian and Maximum likelihood trees for both ITS and trnL-trnF regions and we analysed the genetic distances among the new endemics and the more closely related species. The phylogenetic analyses showed that S. caprariae and S. montis-christi are relatively well separated from S. granulata and more related to S. corsica. This supports the taxonomic treatment previously proposed. We showed that the results of our phylogenetic analysis are in agreement with our biogeographic knowledge of the Tuscan Archipelago and the neighbouring territories and emphasize the conservation relevance of these findings.
Acknowledgements
For help in providing precious material and information, we wish to thank: C. Nepi, Herbarium Centrale Italicum (FI) of University of Florence (Italy); F. Puche, Universidad de Valencia (Spain); M.V. Fornés, Jardí Botànic de Sóller (Spain); Ana Isabel D. Correia, Universidade de Lisboa (Portugal); the Herbarium of University of Copenhagen (C) (Denmark); the Real Jardín Botánico (MA) (Spain); the Museu Nacional de História Natural e da Ciência (LISU) (Portugal); M. Mannocci and V. Mazzoncini of Gruppo Botanico Livornese - Museo di Storia Naturale del Mediterraneo, Livorno (Italy).
Disclosure statement
No potential conflict of interest was reported by the authors.
Supplemental data
Supplemental data for this article can be accessed at http://dx.doi.org/10.1080/14772000.2018.1492997