Cytotoxic Effect and Electrophysiological Study on Human Mrc-5 Fibroblasts of R-Irniine, A Natural Alkylpyrrolidine Alkaloid

Abstract

R-irniine is a natural 2-alkylpyrrolidine alkaloid extracted from Arisarum vulgare Targ., a species belonging to the Araceae family known in Morocco for its toxicity. Cytotoxic and electrophysiological studies of this compound on human MRC-5 fibroblasts were carried out.

Cytotoxic potential of R-irniine was detected on MRC-5 fibroblasts at 40 µg/mL. We carried out the electrophysiological study of this compound on MRC-5 cells by using the technique of patch-clamp in “Whole cell” configuration. R-irniine showed no effect on the outward potassium current of the MRC-5 fibroblasts.

Keywords:

• R-irniine
• Arisarum vulgare
• Araceae
• Cytotoxic effect
• Electrophysiological activity
• MRC-5 Fibroblasts
• Patch-clamp

Acknowledgment

This work is dedicated to Professor Maurice Buclon for all his research work on the Patch-clamp technique.

Notes

Abbreviations: DMSO: Dimethylsulfoxide, EGTA: Ethylene-bis(oxyethylenenitrilo)-tetra-acetic acid, HEPES: [4-(2-hydroxyethyl)-1-piperazine-ethane sulfonic acid]

Additional information

Notes on contributors

Ahmed Melhaoui

Abbreviations: DMSO: Dimethylsulfoxide, EGTA: Ethylene-bis(oxyethylenenitrilo)-tetra-acetic acid, HEPES: [4-(2-hydroxyethyl)-1-piperazine-ethane sulfonic acid]