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Natural Product Research
Formerly Natural Product Letters
Volume 26, 2012 - Issue 11
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Research Articles

Antimicrobial activities of phenethyl isothiocyanate isolated from horseradish

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Pages 1016-1021 | Received 12 Apr 2010, Accepted 17 Sep 2010, Published online: 05 Aug 2011
 

Abstract

Phenethyl isothiocyanate (PEITC) was obtained from horseradish. The preparation procedure was as follows: the horseradish powder was hydrolysed in the water first, and then, after filtration, the residue was extracted by petroleum ether; finally, PEITC was isolated by silica gel column. The structure of PEITC was identified by IR, MS, 1H-NMR and 13C-NMR chromatography methods. The inhibitory activities of PEITC against Gibberella zeae, Xanthomonas axonopodis pv. citri, Cytospora sp. and Phytophthora capsisi showed that PEITC had good inhibition effects. The EC50 values of G. zeae, X. axonopodis pv. citri, Cytospora sp. and P. capsisi were 13.92, 1.20, 0.73 and 3.69 µg mL−1, respectively.

Acknowledgement

This research is supported by the research of NSFC No 2003BAD06B09. The authors thank Mr Jian Xin and Li Bang of Nanjing Agricultural University.

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