Protective role of the triterpenoid-enriched extract of Trichosanthes dioica root against experimentally induced pain and inflammation in rodents

Abstract

Trichosanthes dioica Roxb. (Cucurbitaceae), called pointed gourd in English, is a dioecious climber grown in the Indian subcontinent. This study evaluated the anti-nociceptive and anti-inflammatory effects of triterpenoid-enriched extract of T. dioica root (CETD) in rodents at the doses of 50 and 100 mg kg⁻¹ body weight po. Anti-nociceptive activity was evaluated by acetic acid-induced writhing and tail flick methods in Swiss albino mice. CETD was evaluated for anti-inflammatory activity in experimental acute (carrageenan-, histamine- and serotonin-induced paw oedema) and chronic models (cotton pellet-induced granuloma) in Wistar albino rats. In writhing test, CETD dose dependently and significantly inhibited writhes; in tail flick test, CETD demonstrated significant increase in reaction time (after 60 and 120 min). In all the anti-inflammatory models, CETD exhibited promising anti-inflammatory activity in a dose-dependent manner. Therefore, T. dioica root afforded remarkable anti-nociceptive and anti-inflammatory protections in the tested rodent models.

Keywords:

• Trichosanthes dioica
• anti-inflammatory
• chronic
• anti-nociceptive
• tail flick