Isolation and structure determination of new siderophore tsukubachelin B from Streptomyces sp. TM-74

Abstract

The new siderophore tsukubachelin B (1) was isolated from the iron-deficient culture medium of the newly isolated strain Streptomyces sp. TM-74. The chemical structure of tsukubachelin B (1) was established via interpretation of 2D nuclear magnetic resonance and electrospray ionization-mass spectroscopic data. The structure of tsukubachelin B (1) comprises 6 mol of amino acids, including 2 mol of serine and 1 mol each of ornithine, N-α-methyl-N-δ-hydroxy-N-δ-formylornithine, N-α-methyl-N-δ-hydroxyornithine, and cyclic N-hydroxyornithine.

Keywords:

• siderophore
• Streptomyces
• peptide
• NMR spectrum

Acknowledgements

This study was supported by research funds from the NOVARTIS Foundation (Japan), Noda Institute for Scientific Research, Takeda Science Foundation, Astellas Foundation for Research on Metabolic Disorders, the Foundation of Hattori Hokokai and The Kurata Memorial Hitachi Science and Technology Foundation.