Determination of 10 ginsenosides in Panax ginseng of different harvest times based on HPLC fingerprints and principal component analysis

Abstract

A combinative method of HPLC fingerprinting and quantitative determination was successfully applied to monitor dynamic accumulation of ginsenosides in five-year-old Panax ginseng roots from different harvest times. The optimal chromatographic conditions were achieved on a C18 column with gradient elution using acetonitrile and 1 mmol · L⁻¹ KH₂PO₄ buffer solution at 203 nm. The result indicated that the contents of total ginsenosides showed significant variations, and a decrease tendency appeared in the growth period of the fifth year.

Keywords:

• Panax ginseng
• ginsenosides
• HPLC-DAD
• quantitative determination
• harvest time
• principal component analysis

Acknowledgements

This research work was financially supported by the Key Project of National Natural Science Foundation of China (30830116), and a Project Funded by The Priority Academic Program Development of Jiangsu Higher Education Institutions.