Identification and isolation of non-polar compounds from the chloroform extract of Scutellaria ramosissima

Abstract

In the present study, chloroform extract obtained from aerial parts of Scutellaria ramosissima was phytochemically investigated. A non-polar fraction was obtained from the chloroform extract and the chemical composition of this fraction was identified by GC–MS. The major components of the non-polar fraction of S. ramosissima were determined as heneicosane (12.18%), palmitic acid (11.79%), acetovanillone (6.28%), 5,6-dihydroxy-7,8-dimethoxyflavone (31.87%), (9Z)-octadecenoic (oleic) acid (8.21%), stigmasterol (2.68%), β-sitosterol (2.65%) and 5,2′-dihydroxy-6,7,8,6′-tetramethoxyflavone (2.13%). In addition, 5,6-dihydroxy-7,8-dimethoxyflavone was isolated from the same fraction by PTLC.

Keywords::

• skullcap
• Scutellaria ramosissima
• GC–MS
• PTLC
• flavone

Acknowledgement

This work was supported by UNESCO-L'ORÉAL foundation.