Specific 12α-hydroxylation of grandiflorenic acid by permeabilised fungus Fusarium graminearum

Abstract

Biotransformation of grandiflorenic acid by permeabilised fungus Fusarium graminearum to yield its hydroxylation derivative, 12α-hydroxygrandiflorenic acid, was studied. The biotransformed product was isolated by column chromatography and its structure was determined by mass spectrum and nuclear magnetic resonance analysis. Grandiflorenic acid was efficiently metabolised by the fungus. After 72 h, the substrate was almost completely converted into the product.

Keywords::

• biotransformation
• hydroxylation
• grandiflorenic acid
• 12α-hydroxygrandiflorenic acid; Fusarium graminearum

Acknowledgements

This project was supported by the Program for Excellent Young Teachers of Henan Province (2009GGJS-031). The authors thank Professor Zhiwu Zhu for the English edition of this manuscript.