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Abstract
The increased content of isoflavonoids in dry cell suspension and nutrient medium was observed after application of electric current and AgNO3 on Genista tinctoria L. cultures in vitro. The highest content of genistin (1.7 mg g− 1 DW – dry weight) was measured in the dry cell suspension culture after 30 min elicitation of 10 V and 6 h cultivation and daidzein content (3.5 mg g− 1 DW) was measured after 60 min elicitation of 5 V and 24 h cultivation. In the case of AgNO3 elicitation, the content of genistin in dry cell suspension culture (0.5 mg g− 1 DW) was highest after 48 h of AgNO3 treatment and concentration of 5.9 × 10− 4 mol/L. The AgNO3 concentration of 5.9 × 10− 4 mol/L was also the most effective combination for daidzein production (0.9 mg g− 1 DW) after 168 h. The results of this study show that the secondary metabolites could also be released from G. tinctoria L. cells into the nutrient medium.
Acknowledgements
This work was supported by the (Charles University) SVV project under Grant [2014-260 065]; (The Education for Competitiveness Operational Programme (ECOP) and co-financed by the European Social Fund and the state budget of the Czech Republic) under ‘Support of Establishment, Development, and Mobility of Quality Research Teams at Charles University’ Grant [CZ.1.07/2.3.00/30.0022]. Special thanks should be given to Mrs Miroslava Tichá and Mrs Markéta Šimůnková for their technical support.