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Abstract
Enterobacter cloacae is a versatile bacterial species inhabiting a wide variety of niches and is capable of metabolising a wide variety of substances as energy resources. The fermentation culture of this bacterial species has successfully yielded one new compound, Rimboxa (1) and three known compounds, i.e. indole-3-carboxaldehyde (2), indole-3-acetic acid (3) and 3,4-di-t-butylaniline (4). Rimboxa (1) is shown to possess the 1,2-oxathiolane core structure. 3,4-Di-t-butylaniline (4) is isolated for the first time from a natural resource. These compounds were isolated and characterised using extensive chromatographic and spectroscopic methods, and were subjected to cytotoxicity evaluations.
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Funding
This work was supported by the University of Malaya UMRG [grant number RG094/10AFR], [grant number RP002A-13Bio]; University of Malaya HIR [grant number UM.C/625/1/HIR/043]; Government of Malaysia MOSTI E-science fund [grant number SF004-2014] and Postgraduate Research Fund of University Malaya [grant number PG027-2012B].