Three new phenanthrenone constituents from Trigonostemonlii

Abstract

Three new phenanthrenone constituents, trigoxyphins U–W (1, 7 and 9), together with eight known ones, trigoxyphin M (2), 6,9-O-dimethyltrigonostemone (3), trigonstemone (4), thrigonosomone B (5), trigonochinene E (6), actephiiol A (8), epiactephilol A (10) and neoboutomannin (11), were obtained from the methanol extract of the leaves and stems of Trigonostemonlii. The structures of the new metabolites were elucidated by analysing the spectroscopic data (1D NMR, 2D NMR, HR-ESI-MS and IR). Compounds 1–6 were evaluated for their cytotoxic activities on five human tumour cell lines by using the MTT method, and compound 1 exhibited inhibitory activity against HL-60, SMMC-7721, A-549, MCF-7 and SW480 with IC₅₀ values ranging from 3.77 to 14.51 μM.

Keywords::

• Euphorbiaceae
• Trigonostemonlii
• phenanthrenone
• trigoxyphins U–W
• cytotoxic activity

Acknowledgements

The authors thank Prof. Y. Li (Kunming Institute of Botany, CAS) for providing cytotoxicity test.

Additional information

Funding

This work was supported financially by the National Natural Science Foundation of China [grant number 30830114] and [grant number 21072199], the Ministry of Science and Technology of China [grant number 2009CB522300] and [grant number 2009CB940900] and the Natural Science Fund of Yunnan Province [grant number 2009CD112].