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Abstract
The redox properties of natural extract from cuttlefish ink sac (Sepia officinalis) and synthetic melanin used as a biomimetic in melanin structural investigation were determined by comparison of this phenol-based heterogeneous pigment with gallic acid used as a standard in Folin–Ciocalteu colorimetric assay widely employed for characterisation of oxidative properties of biomaterials. Reactivity of sepia melanin reported here is much higher than previously indicated and this protocol should allow the redox characterisation of all melanins irrespective of their origin and composition.
Graphical abstract
Disclosure statement
No potential conflict of interest was reported by the authors.
Funding
This work was supported by the European Union’s Seventh Framework Programme (FP7/2007-2013) [grant agreement number REGPOT-CT2012-316331-POLARIS]; Khon Kaen University grant to PS.
