Identification and characterisation of serine protease inhibitors from Araucaria angustifolia seeds

Abstract

Araucaria angustifolia seeds are characterised by a relatively high content of starch and protein. This study aimed to verify the presence of α-amylase inhibitors in the seeds and to characterise a trypsin inhibitor found in the embryo tissues. Inhibitor purification was carried out by the saline extraction of proteins, acetone precipitation and affinity chromatography. Two protein bands of molecular weight estimated by SDS-PAGE at about 35 kDa were further examined by high-performance liquid chromatography coupled to a mass spectrometer and were shown to be 36.955 Da (AaTI-1) and 35.450 Da (AaTI-2). The sequence of the N-terminal region shows that AaTI-1 and AaTI-2 are structurally similar to plant inhibitors of the serpin family. A mixture of AaTI-1 and AaTI-2, identified as AaTI, shows selectivity for the inhibition of trypsin (K_iapp 85 nM) and plasmin (K_iapp 7.0 μM), but it does not interfere with the chymotrypsin, human plasma kallikrein, porcine kallikrein or other coagulation enzymes activity.

Keywords:

• Araucaria angustifolia
• serpin
• trypsin inhibitor
• protein purification
• chromatography
• human plasmin inhibitor

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Proc. 2009/53799-5); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNP_q); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).