A new anti-proliferative acylated flavonol glycoside from Fuzhuan brick-tea

Abstract

Fuzhuan brick-tea (FBT) is unique for a fungal fermentation stage in its manufacture process and is classified in dark tea. A new acylated flavonol glycoside, kaempferol 3-O-[E-p-coumaroyl-(→2)][α-l-arabinopyranosyl-(1→3)][α-l-rhamnopyranosyl(1→6)]-β-d-glucopyranoside, which was trivially named as camellikaempferoside A (1), was isolated from FBT along with camelliquercetiside C (2). Their structures were unambiguously elucidated by combination of spectroscopic and chemical methods. Compound 1 showed anti-proliferative activity against MCF-7 and MDA–MB-231 cells with IC₅₀ values of 7.83 and 19.16 μM, respectively.

Keywords:

• Fuzhuan brick-tea
• dark tea
• camellikaempferoside A
• anti-proliferative activity
• MCF-7 cells
• MDA–MB-231 cells

Supplemental data

Supplemental data for this article can be accessed at http://dx.doi.org/10.1080/14786419.2015.1136911.

Acknowledgments

We thank Mr. Xin-Yi Liu and Mr. Mao-Qiang An of Yiyang Fu Cha Industry Development Co. Ltd, for supplying of the tea samples, and Mr. Yu-Song Wang, University of Science and Technology of China, Chinese Academy of Sciences, for NMR measurements.

Funding

This work was supported by National Modern Agriculture Technology System [grant number CARS-23]; National Natural Science Foundations of China [grant number 31300426], [grant number 31101335], [grant number 31471720].