Investigation of in vitro cytotoxic, mutagenic and anti-mutagenic effects of shirazolide extracted from Jurinea leptoloba

Abstract

Shirazolide is an elemanolide isolated from Jurinea leptoloba DC. The aim of this study was to determine the cytotoxic, mutagenic and anti-mutagenic properties of shirazolide from J. leptoloba DC in vitro. Cytotoxicity was measured using a modified MTT (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyltetrazolium bromide) assay on normal human lymphocytes and tumor HeLa cells, showing that the cytotoxicity of shirazolide is much higher for HeLa cells than for normal lymphocytes. Mutagenic and anti-mutagenic activities of shirazolide were evaluated using the Salmonella typhimurium tester strains TA98 and TA100 showing anti-mutagenic properties against the former strain under metabolic activation.

Keywords:

• Shirazolide
• cytotoxicity
• anti-mutagenic
• Salmonella typhimurium

Acknowledgements

. I would like to thank sincerely Dr Iraj Rasooli in the medicinal plant research center of Shahed University for scientific assistance. The authors would like to thank sincerely Dr Mozaffarian for assistance in the botanical information.

Funding

This study was supported by Islamic Azad University’s (Takestan Branch) Research Deputy Office [grant number TIAU: 50155].