Abstract
Phytochemical investigation of Encephalartos villosus Lehm. leaves afford two new illudalane sesquiterpenes namely Encephaldiene 1 and Encephaldiene 2 together with four known flavone glycosides, Luteolin-7-rutinoside, Luteolin-7-glucoside, Luteolin-7-rhamnoside and Apigenin-7-glucoside. The structures of the isolated compounds were elucidated by means of spectroscopic methods including 1D and 2D NMR experiments along with HRESIMS spectrometry. Antimicrobial activity of CHCl3 and MeOH extracts was investigated. Both extracts showed antibacterial activity against Gram-positive bacteria Streptococcus pneumonia and Bacillus subtilis, and antifungal activity against Aspergillus fumigatus. While CHCl3 extract showed additional activity against Gram-negative bacteria Escherichia coli.
![](/cms/asset/48d94473-b7c1-4c1c-be9d-c6f6deaf9d01/gnpl_a_1160236_uf0001_oc.jpg)
Acknowledgement
The authors would like to thank the Regional Center of Mycology and Biotechnology for antimicrobial tests.