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A new 5(6→7)abeo-sterol with rarely reported [6–5-6–5]-fused rings, 3β,5β,6-trihydroxyl-B-norsitostane (1), along with 10 known compounds were isolated from the twigs of Podocarpus fleuryi. Their structures were elucidated by means of extensive spectroscopic analysis. Compound 1 was assessed for their cytotoxicity against five human tumour cell lines (HL-60, SMMC-7721, A-549, MCF-7 and SW-480), and the result showed that it had no activity.
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