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Chemical investigation of the stems of Epigynum auritum led to the isolation and identification of a novel 16,17-seco pregnane glycoside, epigynoside D, along with other three known compounds (2–4). The structure of compound 1 was elucidated by means of spectroscopic analysis, including HRESIMS, 1D and 2D NMR experiments. All isolated compounds were tested for their in vitro cytotoxic, immunological and anti-acetylcholinesterase activities.
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