Evaluation of the anticoagulant potential of polysaccharide-rich fractions extracted from macroalgae

Presented to CIPAM 2016, 6th International Congress of Aromatic and Medicinal Plants, 29 May–1 June 2016, Coimbra (Portugal)

Abstract

The aim of this study was to evaluate the potential anticoagulant activity of sulphated polysaccharide-containing extracts of six french edible marine macroalgae. Aqueous extracts of brown (Himanthalia elongata, Laminaria digitata, Ascophyllum nodosum, Fucus vesiculosus), green (Ulva lactuca) and red (Chondrus crispus) macroalgae were prepared and their biochemical properties were determined, including major biomolecules, sulphate and ash contents. The anticoagulant activity of each extract was investigated using different scales from the specific antithrombin-dependent pathway (anti-Xa and anti-IIa) to the intrinsic and/or common (Activated Partial Thromboplastin Time, APTT), extrinsic (Prothrombin Time, PT) or common (Thrombin Time, TT) anticoagulant pathways, and compared with those of commercial anticoagulants, heparin and Lovenox®. Laminaria digitata, Fucus vesiculosus and Chondrus crispus extracts showed a significant APTT anticoagulant capacity, only 5-fold lower than that of Lovenox®, which is a pure low molecular weight heparin used as an anticoagulant agent to prevent pulmonary embolism in patients undergoing surgery.

Keywords:

• Marine macroalgae
• Phaeophyceae
• Chlorophyceae
• Rhodophyceae
• aqueous extracts
• anticoagulant activity

Acknowledgements:

We are very grateful to the hospital of La Rochelle, which kindly provided us Lovenox®, and to the “Biomolecule High Resolution Analysis Platform” from the LIENSs Laboratory of La Rochelle University for the UHPLC-HRMS analyses.

Notes

Presented to CIPAM 2016, 6th International Congress of Aromatic and Medicinal Plants, 29 May–1 June 2016, Coimbra (Portugal)