Abstract
The GC-MS analysis of fractions of methanol extract of stem of Cuscuta reflexa (Family Convolvulaceae) was carried out using a THERMO TRACE 1300 Gas Chromatograph equipped and coupled to a mass detector THERMO TSQ 8000 spectrometer with an TG 5MS (30 m × 0.25 mm, 0.25 μm) of capillary column. Database of National Institute of Standards and Technology (NIST) library was used to identify the components. GC-MS revealed two known compounds i.e. 2-Methoxy-4-vinyl phenol (6.80%) and Benzofuran-2,3-dihydro (20.89%) and other 12 unknown compounds such as 3,5-di-tert-Butyl-4-hydroxyanisole (35.50%); Hexatriacontane (12.02%); n-Hexadecanoic acid (10.12%); Scoparone (7.97%); Hexadecanoic acid methyl ester (5.22%); 1,3-Benzenediamine, N, N, N′, N′ tetramethyl- (15.43%); Phenol, 4(3-hydroxy1propenyl), 2-methoxy (9.31%); Phenol, 2,4 bis (1,1dimethylethyl); 2,3,5,6-Tetramethyl para phenylene diamine (14.18%); Retinoic acid-5,6-epoxy-5,6-dihydro (20.46%); 2,4-Dihydroxy-2,5-dimethyl-3(2H)furan-3-one (10.13%); 2,3-dihydro-3,5-dihydroxy-6-methyl-2-Propyl-tetrahydro-pyran-3-ol (10.13%); Pregn-4-ene-18-oic acid (5.75%) as some of the major compounds in its different fractions. Retinoic acid-5,6-epoxy-5,6-dihydro found as major compound may be responsible for blood glucose lowering potency.
Acknowledgement
We are very much thankful to the Central Instrumentation Laboratory, Sophisticated Analytical Instrumentation Facility (SAIF), Chandigarh, Panjab University, India for their support in conducting GC-MS analysis.