Abstract
Our study aimed to investigate the protective role of SDAPR on cisplatin-induced cytotoxicity and its’ possible mechanism in HEK293 cells. Cell viability was measured by MTT assay. Oxidative stress (SOD, GSH, LDH and MDA), inflammatory factors (TNF-α and IL-6) and apoptosis-related proteins (caspase-3, Bax, Bcl-2) expression were measured. The apoptotic cells were observed by TUNEL staining. Our study results indicated that non-cytotoxic levels of SDAPR significantly increased viability rate (LD50 value of cisplatin is 20 μM), which improved antioxidant defence, attenuated apoptosis by decreasing expression levels of cleaved-caspase-3 and Bax, increasing Bcl-2 expression and inhibiting apoptotic positive cells in HEK 293 cells. In addition, SDAPR treatment markedly inhibited the levels of TNF-α and IL-6. In conclusion, Sika deer antler protein, a potential modulator, could alleviate cisplatin-induced cytotoxicity in HEK 293 cells.
Abbreviations:
- Human embryonic kidney 293 (HEK 293)
- Sika deer antler protein (SDAPR)
- 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
- Dimethyl sulfoxide (DMSO)
- Super oxide dismutase (SOD)
- Glutathione (GSH)
- Lactate dehydrogenase (LDH)
- Malondialdehyde (MDA)
- Bicinchoninic acid (BCA)
- Radio-Immunoprecitation (RIPA)
- Phenymethanesulfonyl fluoride (PMSF)
- Tumour necrosis factor alpha (TNF-α)
- Interleukin-6 (IL-6)
- Sodium dodecyl sulfate (SDS)
- The terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL)