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Natural Product Research
Formerly Natural Product Letters
Volume 33, 2019 - Issue 11
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Research Articles

Extraction and partial characterisation of antioxidant pigment produced by Chryseobacterium sp. kr6

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Pages 1541-1549 | Received 17 Oct 2017, Accepted 27 Dec 2017, Published online: 05 Jan 2018
 

Abstract

Pigments synthesised by Chryseobacterium sp. kr6 growing on feather waste were extracted and characterised. The pigment extract was characterised by KOH test, UV–vis, CIELAB colour system, HPLC-DAD-MS, FTIR and its antioxidant capacity was evaluated. A positive bathochromic shift was observed when kr6 colonies or pigment extracts were subjected to alkaline solution (20% KOH) and a λmax at 450 nm was detected for acetone extracts, although no typical fine structure of carotenoids was detected in the electomagnetic spectra. The HPLC profile of the extracted pigment showed that the compound has three different peaks with λmax near 450 nm. The FTIR analysis shows some principal functional groups from a flexirubin-like molecule. The pigmented compound also presents antioxidant activity evaluated by the scavenging of the ABTS radical.

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