Isolation, purification and identification of two new alkaloids metabolites from marine-derived Verrucosispora sp. FIM06025

Abstract

Chemical investigation of a marine-derived actinomycete strain Verrucosispora sp. FIM06025 isolated from a marine sponge sample collected from the East China Sea, resulted in the discovery of two new alkaloids, (2-(hydroxymethyl)-3-(2-(hydroxymethyl)-3-methylaziridin-1-yl) (2-hydroxyphenyl) methanone (1) and 2-(1-hydroxyethyl)-3,4-dihydrobenzo [f] [1,4]oxazepin-5(2H)-one (2). The structures of compounds 1 and 2 were determined by the detailed analysis of 1D, 2D NMR and HR-TOF-MS data, along with literature data analysis. The bioefficacy investigations revealed that compound 1 exhibited a broad spectrum of antimicrobial activity with MIC (minimum inhibitory concentration) values ranging from 3.4 to 200 μg·mL⁻¹ against H. pylori, P. aeroginosa, A. baumanniiin, E. coli and K. pneumonia, S. aureus, C. albicans and E. faecium, however, compound 2, up to 200 μg/mL, displayed no antibacterial activity against these bacteria.

Graphical Abstract

Keywords:

• Alkaloid
• Verrucosispora
• marine sponge
• antimicrobial activity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This project was financially supported by the National Key R&D Program of China (2018YFC0311001), Fujian Province Natural Science Foundation (grant number 2015J01290), the Fujian Provincial Major Science and Technology Special Project (grant number 2017R1009-7) and the Fujian Province Marine High-tech Project (grant number Min Marine High-tech [2016]25).