A new indole-type alkaloid from the roots of Clematis florida var. plena

Abstract

One new indole-type alkaloid, α-L-rhamnopyranosyl-(1→6)-β-D- glucopyranosyl 6-methoxy-3-indolecarbonate (1), together with three known alkaloids (2–4), one aromatic acid (5) and five known saponins (6–10), was isolated from the roots of Clematis florida var. plena. Their structures were established by NMR spectroscopic analysis and acid hydrolysis. In in vivo anti-inflammatory activity, n-butanol extract was found to be potent against ear edema in mice, with inhibition rate of 48.7% at a dose of 800 mg/kg. Furthermore, compounds 8 and 9 obtained from the n-butanol extract exhibited significant anti-inflammatory activities with inhibition rates of 50.9% and 54.7% at a dose of 200 mg/kg.

Graphical Abstract

Keywords:

• Clematis florida var. plena
• alkaloid
• saponins
• anti-inflammatory

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by the Natural Science Foundation of Fujian Province [grant number 2016J01369], [grant number 2018J01847]; the Open Project of National Marine Bureau Key Laboratory of Marine Biogenetic Resources [grant number HY201807], [grant number HY201604]; the Science and technology innovation platform project of Fujian Province [grant number 2018Y2001].