Terpenoids from the roots of Leontopodium longifolium and their inhibitory activity on NO production in RAW264.7 cells

Abstract

Phytochemical investigation of the roots of Leontopodium longifolium, led to the isolation of a novel norsesquiterpene, named as longifolactone (1), along with three known diterpenes. The structures of these compounds were elucidated by analysis of HR-ESI-MS, UV, IR and 1D and 2D NMR spectroscopic data. The absolute configuration of the new compound was determined by electronic circular dichroism (ECD) using both experimental and calculated ECD spectra. Furthermore, their anti-inflammatory effects were evaluated in LPS-activated RAW264.7 cells to determine their effects on the release of NO. Longifolactone (1) showed weak cytotoxicity towards two human cancer cell lines.

GRAPHICAL ABSTRACT

Keywords:

• Leontopodium longifolium
• Longifolatone
• NO production inhibition
• Sesquiterpene lactones

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Projects of Collaborative Innovation Team in Gansu Department of Education (No. 2017C-10) and the Lanzhou JiaoTong University excellent platform support funding (No. lzjtu20p7 EP support). Hai-Bei Li thanks the fund of Young Scholars Program of Shandong University (Weihai) and the supercomputing system in the Supercomputing Center, Shandong University, Weihai.