A new sesquiterpene, a new monoterpene and other constituents with anti-inflammatory activities from the roots of Aristolochia debilis

Abstract

A new sesquiterpene (1) and a new monoterpene (2), together with thirteen known compounds (3–15) were isolated from an ethanol extract of the roots of Aristolochia debilis Sieb. et Zucc. The structures of compounds 1 and 2 were elucidated using HR-ESI-MS, 1D and 2D NMR spectroscopy. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of nitric oxide, tumour-necrosis factor-α and interleukin-6 production in lipopolysaccharide-stimulated RAW264.7 cells. Compounds 1–9 and 12–15 significantly inhibited the levels of NO, TNF-α and IL-6 in LPS-induced RAW264.7 cells from concentrations of 3 μM to 30 μM.

Keywords:

• Aristolochia debilis
• Aristolochiaceae
• anti-inflammatory
• monoterpene
• sesquiterpene

Acknowledgments

This work was supported by the National Natural Science Foundation of China [grant numbers 81760627, 81660699 and 81660579]. This work was also partially supported by Jilin Provincial Education Department [grant number 2016-280]. We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing an English draft of this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.