The active fraction from the tuber of Bolboschoenus yagara inhibits melanoma B16 cells metastasis LPS-induced in vitro and in vivo

Abstract

This study was to identify anti-metastatic active fractions and compounds of Bolboschoenus yagara (B. yagara). The results indicated that 50 µg/mL ethyl acetate fraction (Et) can dramatically inhibit mouse melanoma B16 cells migration and invasion in vitro. In B16 cells pulmonary and hepatic metastasis assays, 50 µg/mL Et alleviated mouse lung and liver weights, the number of metastatic nodules and the levels of TNF-α and IL-6 in mouse serum and organs. Histological studies showed that Et fraction was able to prevent liver and lung metastasis. And the inhibition of 50 µg/mL Et fraction against hepatic metastasis was almost equivalent to that of 1 µM TAK242. In addition, fourteen compounds of Et were quantified by HPLC analysis, in which, isocoumarins, stilbenes and xanthones obviously abated LPS-modulated B16 cells migration and invasion.

Keywords:

• Bolboschoenus yagara (Scirpus yagara)
• melanoma
• metastasis
• LPS
• isocoumarins

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 81173515 to QL); Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization (ZDXM-2-4); Jiangsu Graduate Research Innovation Program (KYCX18_1638).