Inducing secondary metabolite production from Daldinia eschscholzii JC-15 by red ginseng medium

Abstract

Red ginseng (RG) is one of the most popular herbal medicines and used as a dietary supplement in recent years. The bioactive ingredient in RG can induce the production of novel microbial metabolite from fermented RG. Using the one strain-many compounds strategy, the reinvestigation of the metabolites from Daldinia eschscholzii JC-15 cultured in red ginseng medium led to the isolation of an unprecedented benzopyran-naphthalene hybrid, daldinsin (1) and a new lactone (2). In this research, a new lactone, 8-hydroxylhelicascolide A (2) instead of helicascolide A was produced by the D. eschscholzii JC-15 induced by the red ginseng medium. Compound 1 showed anti-acetylcholinesterase activity with the inhibition ratio of 38.8% at 50 μM. Compound 2 indicated antimicrobial activities against Fusarium Solani, F. oxysporum, and Escherichia coli with MICs at 128 μg/mL. RG is therefore a promising activator in production of novel microbial metabolite.

Graphical Abstract

Keywords:

• Metabolite
• Daldinia eschscholzii
• red ginseng
• medium induction
• bioactivity

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This research work was financially supported by the National Natural Science Foundation of China (81660582, 81560571), and a project of Yunling Scholars of Yunnan Province.