Isolation of cytotoxic monomeric protein and morin derivatives from Solena amplexicaulis (Lam.) Gandhi

Abstract

Solena amplexicaulis (Lam.) Gandhi (family- Cucurbitaceae), is used both in the Indian traditional system and folk medicine to treat several pathophysiological conditions and complex diseases including cancer. The screening of the phytochemicals of this plant (aerial parts) was performed to evaluate their cytotoxic effect against an in vitro cancer model utilising acute promyelocytic leukaemia HL60 cell line. Phytoconstituents were isolated by column chromatography and characterised. The purified protein was extracted, isolated and purified by using standard techniques. The cytotoxicity was evaluated by MTT assay. Spectral analysis revealed the isolated phytochemicals to be Morin-3-O-xyloside (1) and Morin 3-O-glucoside (2). The purified protein (P1) was found to be monomeric having a molecular weight of 30.2 kDa. Watching over 24 h exposure, compound 1 (IC₅₀ 1.5 µmol/L), compound 2 (IC₅₀ 3.5 µmol/L), and P1 (2.67 µmol/L) exhibited significant cytotoxic activity.

GRAPHICAL ABSTRACT

Keywords:

• Oncology
• flavonoids
• herbs
• in vitro
• cell culture

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors would like to thank Jadavpur University, Kolkata, India, for providing research facilities and this study was supported by University Grants Commission, New Delhi, India, 2012.