Anti-inflammatory effect and mechanism of action of ellagic acid-3,3′,4-trimethoxy-4′-O-α-L-rhamnopyranoside isolated from Hopea parviflora in lipopolysaccharide-stimulated RAW 264.7 macrophages

Abstract

Phytochemical investigation of the stem bark of Hopea parviflora resulted in the isolation of 9 compounds; which includes friedelin (1), friedelin-3β-ol (2), (-)-ampelopsin A (3), (-)-ɛ-viniferin (4), (-)-hopeaphenol (5), vaticaphenol A (6), 2,4,8-trihydroxyphenanthrene-2-O-glucoside (7), ellagic acid-3,3’,4-trimethoxy-4’-O-α-L-rhamnopyranoside (8) and β-sitosterol-β-D-glucoside (9). Among them, compounds 1, 2, 6, 7, 8 and 9 are isolated for the first time from this species. Further, we evaluated the anti-inflammatory activity of compounds 4, 5, 6, 7 and 8. In this study, compound 8 inhibited the activity of proinflammatory mediators like NO, TNF-α, IL-6, 5-LOX and COX-2, also promoted the action of anti-inflammatory mediator like IL-10 via inhibition of the NF-κB pathway in LPS-stimulated RAW 264.7 macrophages.

Graphical Abstract

Keywords:

• Hopea parviflora
• Dipterocarpaceae
• ellagic acid-3,3',4-trimethoxy-4'-O-α-L-rhamnopyranoside
• anti-inflammatory effect

Acknowledgements

B. P, T. S. P, P. S and G. G. thank CSIR and UGC for research fellowships. S.S thank SERB-DST for post-doctoral fellowship.

Disclosure statement

The authors confirm that this article content has no conflict of interest.

Additional information

Funding

This work was supported by the Department of Science and Technology (DST), New Delhi (Grant number SR/S1/OC-24/2014).