Bioactive chemical constituents from Curcuma caesia Roxb. rhizomes and inhibitory effect of curcuzederone on the migration of triple-negative breast cancer cell line MDA-MB-231

Abstract

Rhizomes of Curcuma caesia are traditionally used to treat cancer in India. The aim is to isolate chemical constituents from C. caesia rhizomes through bioassay-guided fractionation. The extract, hexanes and chloroform fractions showed effect on MCF-7 and MDA-MB-231cells in cell viability assay. The chromatographic separation afforded germacrone (1), zerumbone (2), furanodienone (3), curzerenone (4), curcumenol (5), zederone (6), curcumenone (7), dehydrocurdione (8) from hexanes fraction and curcuminol G (9), curcuzederone (10), (1S, 10S), (4S,5S)-germacrone-1 (10), 4-diepoxide (11), wenyujinin B (12), alismoxide (13), aerugidiol (14), zedoarolide B (15), zedoalactone B (16), zedoarondiol (17), isozedoarondiol (18) from chloroform fraction. This is first report of compounds 2, 9–13, 15–18 from C. caesia. The study demonstrated compounds 1–4 and 10 are the bioactive compounds. The effect of curcuzederone (10) on MDA-MB-231 cell migration showed significant inhibition in scratch and Transwell migration assays. The results revealed that curcuzederone could be a promising drug to treat cancer.

Graphical Abstract

Keywords:

• Curcuma caesia Roxb.
• cell viability assay
• bioactive chemical constituents
• cell migration
• curcuzederone
• breast cancer cell lines

Acknowledgements

The authors thank to Ms. Hosne Ara for the authentication of plant.

Disclosure statement

The authors declare that there is no potential conflict of interest.

Additional information

Funding

The study was supported by Universiti Sains Malaysia (RUI grant: 1001/PFARMASI/8011003).