Potential application of berberine in the treatment of Escherichia coli sepsis

Abstract

Gram-negative sepsis ranks as the leading cause of death in intensive care units. Despite the development of new antibiotics, mortality from gram-negative sepsis remains high. The present study aims to investigate the in vivo effects of berberine (BBR) administration on septic death induced by intraperitoneal Escherichia coli injection. The results showed that (i) single 5 mg/kg dose of BBR increases the survival of septic mice, (ii) BBR administration improves the antimicrobial efficacy of antibiotic drug, (iii) BBR pre-treatment prevents improvements of BBR therapy without affecting the pro-survival effects of antibiotic drug. The effects of BBR administration were associated with immunological alterations represented by changes in CD4+ and CD8+ lymphocytes population and IL-6 and TNF-α production. This study highlighted the benefits of berberine administration as antibiotic adjuvant in E. coli sepsis. Furthermore, information about berberine-induced immunological perturbations and their influence on host response to infection and therapy has been shown.

Graphical Abstract

Keywords:

• Berberine
• Escherichia coli
• sepsis
• immunomodulation
• animal model
• IL-6
• TNF-α
• CD4
• CD8

Acknowledgements

The authors wish to thank Ms. Beatrice Bartozzi and Ms. Claudia Giaccaglia for their technical assistance.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

This study was partially supported by Ricerca Corrente funding from Italian Ministry of Health to IRCCS INRCA.