In vitro and in vivo evaluation of anti-inflammatory activity and free radical scavenging potential of leaves extract from Anadenanthera colubrina

Abstract

The present study highlights the antioxidant and anti-inflammatory activities from Anadenanthera colubrina leaves ethanolic extract (EEAc) and its phytochemical profile. The chemical profile of EEAc was determined and in vitro free radical scavenging potential, cell viability in RAW 264.7 and in vitro and in vivo anti-inflammatory activity were evaluated. The analysis of EEAc showed several phenolic compounds such as tannins and phenolic acids. The high antioxidant potential observed is possibly due to its high phenolic content. The EEAc (500 mg kg⁻¹) showed an in vivo effect at the same level of dexamethasone; in vitro, at 50 μg mL⁻¹, inhibited approximately 80% of nitric oxide production concentration, showed an inhibition of more than 50% of TNF-α production and presented high cell viability. The results show that A. colubrina leaves are an important source of phytochemicals that possesses antioxidant and anti-inflammatory properties.

Graphical Abstract

Keywords:

• Antioxidant
• capillary electrophoresis
• IL-10
• IL-12
• NMR spectroscopy
• phenolic compounds
• cytokine

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

We are grateful to Brazilian research agencies CAPES, CNPq (460184/2014-8) and FAPEMIG (MPR-00262-16; APQ02423-18) for the financial support.