Lathyrane diterpenoids from Jatropha podagrica and their antitumor activities in human osteosarcoma cells

Abstract

Two new lathyrane-type diterpenoids, jatropodagins A and B (1 and 2), and five known analogues (3–7), were isolated from the stems of Jatropha podagrica. Their structures and absolute configurations were elucidated by spectroscopic data and calculated ECD analyses. The cytotoxicities of all the lathyrane-type diterpenoids (1–7) were evaluated against two human osteosarcoma cell lines (Saos-2 and MG-63). Compound 1 exhibited significant cytotoxic effects against Saos-2 and MG-63 with IC₅₀ values of 8.08 and 14.64 μM, respectively. The IC₅₀ values for the positive control 5-FU against the Saos-2 and MG-63 cell lines were 19.01 and 25.00 μM, respectively. Morphological features of apoptosis activities were evaluated in 1-treated Saos-2 cells and the results confirmed apoptosis in a dose-dependent manner.

Graphical Abstract

Keywords:

• Jatropha podagrica
• lathyrane
• diterpenoid
• antitumor activity

Disclosure statement

No potential conflict interest was reported by the authors.

Additional information

Funding

This work was supported by the National Natural Science Foundation of the People’s Republic of China (81703672); Pudong New Area Health Commission (PDZY-2018-0610 and PWRd2018-03); Clinical Specialty Project of Traditional Chinese Medicine in Pudong New Area of Shanghai (PDZY-2018-0612); Clinical Chinese Medicine Plateau Discipline Construction Project of Shanghai Pudong New Area Health Committee (PDZY-2018-0604); Projects within the Budget of Shanghai University of TCM (18LK066); Shanghai Health Committee (20194Y0424); Science and Technology Development Fund of Shanghai Pudong New Area (PKJ2019-Y18); the Pudong New Area Special Research and Innovation of Chinese Medicine (PDZY-2018-0801) and the Outstanding Clinical Discipline Project of Shanghai Pudong (PWYgy2018-05).