Metabolite profiling of Andrographis paniculata leaves and stem extract using UHPLC-Orbitrap-MS/MS

Abstract

Andrographis paniculata is known as the king of bitter and it has been widely used as a medicinal plant. The properties of A. paniculata are generally determined by the metabolite composition, which may be influenced by several factors, one of which is the part of the plant extracted. The objectives of this research are to identify putatively the metabolite composition of the stem and the leaves extracts using LC-MS/MS and classify them using PCA. The stem and the leaves samples were separated and extracted using sonication with 70% ethanol. A total of 31 metabolite compounds has been putatively identified. All compounds were identified in the stem and the leaves extracts, which only differed in their intensity. These metabolite compounds were divided into diterpene lactones, flavonoids, and phenolic acid groups. By using the peak intensities of the 18 compounds identified, the leaves and stem extracts were grouped using PCA.

Keywords:

• Andrographis paniculata
• LC-MS/MS
• metabolite profiling

Disclosure statement

There is no conflict of interest.

Additional information

Funding

The authors gratefully acknowlegded Ministry of Research, Technology, and Higher Education of the Republic of Indonesia through World Class University Program of IPB University for financial support of this research by Riset Kolaborasi Indonesia Grant 2019 (No: 0847/IT3.L1/PN/2019).