Anti-inflammatory activity of glycolipids isolated from cyanobacterium Nodularia harveyana

Abstract

Activity-guided fractionations from the freshwater cyanobacterium Nodularia harveyana led to the isolation of two monogalactosyldiacylglycerols (MGDG), two digalactosyldiacylglycerols (DGDG), two monoglucosyldiacylglycerols (MGlcDG) and 1-(O-hexose)-3,25-hexacosanediol (HG). Structures were elucidated by a combination of 1D and 2D NMR analysis, HRMS and GC-MS. The potential for inhibition against TNF-α and NF-κB production of these seven compounds was tested in THP-1 cells. All compounds showed activity, but compound 7 showed higher inhibitory activity of TNF-α and NF-κB, with IC₅₀ of 4.88 ± 0.13 and 3.64 ± 0.45 μM, respectively.

Graphical Abstract

Keywords:

• Cyanobacterium
• Nodularia harveyana
• glycolipids
• anti-inflammatory
• TNF-α
• NF-κB

Supplementary material

Supplementary material relating to this paper is available online. NMR and GC-MS spectra for compounds 1–7 (Figures 1S–13S) is provided as supporting information.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was supported by the Fundación de la Universidad Autónoma de Madrid.