New pyran derivative with antioxidant and anticancer properties isolated from the probiotic Lactobacillus plantarum H24 strain

Abstract

This work was aimed to prepare, isolate and identify antioxidant and cytotoxic compound from the culture filtrate of a probiotic lactobacillus strain. New compound, plantarone (1), together with two known compounds, kojic acid (2) and methyl dodecanoate (3), were isolated from the ethyl acetate extract of the culture filtrate of probiotic Lactobacillus plantarum H24. Their structures were elucidated using spectroscopic methods including 2 D NMR, HRMS analyses. Isolated compounds were screened for antioxidant and cytotoxic activities against Caco-2 colon cancer cells. Compounds 1 and 2 showed lowers DPPH radical scavenging activities (p < 0.05) with IC₅₀ values of 66.3 ± 0.34 μM and 50.2 ± 0.28 μM respectively, compared to standard butylated hydroxyanisole (BHA: IC₅₀ = 44.2 ± 0.24 μM). Whereas only compound 1 showed a good cytotoxicity activity with inhibition value of 60.72 ± 3.55%. Accordingly, L. plantarum H24 could be used to prevent oxidative stress and its injuries, improving human health.

Graphical Abstract

Keywords:

• Antioxidant activity
• Spectroscopy
• cytotoxic activity
• Lactobacillus plantarum H24
• plantarone
• probiotics

Acknowledgements

We are grateful to the Third World Academy of Sciences (TWAS) and the International Center of Chemical and Biological Sciences (ICCBS), University of Karachi, Pakistan.

Disclosure statement

The authors declare no competing interests

Additional information

Funding

This research was financed by the Third World Academy of Sciences (TWAS) and International Center of Chemical and Biological Sciences (ICCBS), University of Karachi, Pakistan; through two of the authors: H.C.K.M [grant FR number: 3240299154] and A.D.K.M [grant FR number: 3240299153].