Abstract
Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). There are no available drugs due to the emergence of variant of PRV. Dihydromyricetin (DMY) is a flavonoid extracted from Ampelopsis grossedentata (A. grossedentata), which has a variety of pharmacological activities. In this study, we aim to investigate the in vitro anti-PRV activity of DMY extracted from A. grossedentata. MTT assay was used to detect the cytotoxicity and antiviral activity of DMY. The results detected by flow cytometry and qRT-PCR showed that DMY played anti-PRV role mainly by interfering with the process of virus invasion into host cell and inhibiting the occurrence of pyroptosis in vitro. This suggested that anti-pyroptosis may be an important antiviral mechanism for DMY which is expected to be a potential anti-PRV drug.
Graphical Abstract
![](/cms/asset/82fd0f04-8de2-4195-a750-8741b9ad5ca8/gnpl_a_1982935_uf0001_c.jpg)
Disclosure statement
No potential conflict of interest was reported by the authors.