Separation of a new triterpenoid saponin together with six known ones from Clematis tangutica (Maxim.) Korsh and evaluation of their cytotoxic activities

Abstract

A new triterpenoid saponin, 3-O-β-D-allopyranosyl (1→3)-α-L-rhamnopyranosyl (1→2)-α-L-arabinopyranosyl hederagenin 28-O-α-L-rhamnopyranosyl (1→4)-β-D-glucopyranosyl (1→6)-β-D-glucopyranosyl ester (IV), together with six known ones Hederacholichiside F (I), Tanguticoside B (II), Tauroside St-H₁ (III), Hederoside H₁ (V), Kalopanaxsaponin G (VI), Hederasaponin B (VII) were separated from Clematis tangutica (Maxim.) Korsh. Their cytotoxic activities were evaluated. Saponins IV (new compound) and I showed selective inhibitory activities against HGC-27 with IC₅₀ values of 20.17 and 66.18 μM. Saponin VII exhibited extensive inhibitory action against HGC-27, Hela and SK-OV-3 with IC₅₀ values of 16.47-71.36 μM. Saponin III showed selective inhibitory activity against SK-OV-3 with the IC₅₀ value of 48.70 μM. All isolated saponins were inactive (IC₅₀ >150 μM) to GES-1.

Graphical Abstract

Keywords:

• Clematis tangutica (Maxim.) Korsh
• triterpenoid saponins
• new compound
• high-speed counter-current chromatography
• cytotoxic activities

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Innovation Platform Construction Project of Qinghai Province [2020-ZJ-T02]; Key Research, Development and Transformation Project of Qinghai Province [2019-SF-123]; Innovation Capability Improvement Project for Colleges and Universities of Gansu Province [2019A-102]; Surface Project of Key Laboratory of Hexi Corridor Resources Utilization of Gansu [XZ1805]; and Joint Research Project of Three-River Headwaters National Park, Chinese Academy of Sciences and The People’s Government of Qinghai Province [LHZX-2020-09].